In vitro functional screening
IN VITRO EFFICACY COMPOUND PROFILING
CANCER STEM CELLS SHOW THE UNIQUE ABILITY TO GROW AS FLOATING SPHEROIDS WHEN SEEDED IN SUSPENSION WITH DEFINED CULTURE MEDIA.
Tumor shaped spheroid 3D cell culture systems allow stronger, more efficient and reliable drug screening results than conventional 2D based monolayer culture systems currently in use. This assay is optimized for breast, colon, lung, ovarian, prostate, pancreas and glioblastoma cell lines, and can also accommodate others. We carry out a toxicity pre-screening to discard non-specific cytotoxics, aiming to uncover specific molecules targeting the cancer stem cell compartment.
We specialize in evaluating the effect of drug candidates on the standard cancer stem cell markers and assays, such as surface marker expression or ALDH activity determination.
Characterization of stem cell related gene signatures, where we measure a panel of genes related to identification, homeostasis, growth and differentiation of stem cells. Allows the evaluation of potential leads in self-renewal and differentiation potential of stem cells, critical parameters for cancer progression.
Cancer stem cells are responsible for initiating new tumor lesions in distal locations that may eventually lead to a metastasis. Therefore it is critical to monitor the ability of cancer stem cells to migrate as a measure of invasive capacity, thus a surrogate marker for metastatic potential. StemTek can measure the ability of spheroid derived cells to migrate on extra cellular matrix covered plates and observe the speed of 2D growth as a measure of invasive potential of these cells.
Using Cell2Sphere™ technology.
Modified Xenograft models to assess tumor initiation.
Increased physiological relevance for compound profiling.